IUCN / SSC Cat Specialist Group - Digital Cat Library
   

 

View printer friendly
Faizah, U.
Karakteristik marka genetik daerah D-loop bagian HVS-I sebagaiacuan konservasi genetik harimau sumatera
2009  Conference Proceeding

Numbers of Sumatran tiger (_Panthera tigris sumatrae_) are currently declining and its existence is in danger. Therefore, efforts should be taken for its conservation, including genetic conservation. Displacement loop (D-loop) in mitochondrial DNA (mtDNA) has been widely used to study the genetic diversity and kinship of various sub-species. The aims of this study were (1) to analyze the genetic diversity of genetic markers on the D-loop region HVS-I part; (2) to determine the relationship of kinship between individuals of Sumatran tiger and with other tiger subspecies in the world. The research method is amplification by PCR in the area of the D-loop section HVS-I using a primger UF-03 and UF-04 (PCR product of 567 bp). Furthermore, the process of sequencing and data sequencing results were analyzed using the program MEGA IV using data from Genbank as a comparison. Phylogeny was reconstructed using Neighbour-Joining (NJ) with 1000 repetitions. The analysis of genetic diversity showed 15 nucleotide sites specific in Sumatran tigers (24 (G), 74 (A), 76 (A), 136 (C), 138 (C), 179 (C), 212 (A), 302 (G), 318 (T), 395 (C), 406 (G), 417 (A), 430 (A), 484 (G), 488 (G)). Kinship analysis between individuals showed that in this study Sumatran tigers from Jambi were closer to those from Riau compared with Sumatran tiger from Medan. The subspecies is closest related to the Bengal tiger, and farthest from the South China tiger. The conclusion from this study is the genetic marker D-loop section HVS-I is suitable for distinguishing between subspecies and between individuals within a group of Sumatran tigers.

PDF files are only accessible to Friends of the Cat Group. Joining Friends of the Cat Group gives you unlimited access and downloads in the Cat SG Library for one year, and allows you to receive our newsletter Cat News (2 regular issues per year plus special issues). More information how to join here

 

(c) IUCN/SSC Cat Specialist Group ( IUCN - The World Conservation Union)