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Faizah, U.
Karakteristik Marka Genetik DNA Mitokondria Sebagai Acuan Konservasi Genetik Harimau Sumatera
2008  Full Book

Sumatran tiger (_Panthera tigris sumatrae_) is the only surviving tiger subspecies living in Indonesia and is now gravely threatened. Knowing the genetic status and genetic diversity of a population can aid in designing a program to avoid extinction and help the development of management plans for survival (Damayanti 2007). Assessment of genetic diversity through molecular tagging using Deoxyribonucleic Acid (DNA), both from nuclear DNA and mitochondrial DNA (mtDNA) will reveal differences on a intra- and interspecies level concerning the structure, composition, and genome organization at the level of DNA (Duryadi 1994). Cytochrome b (Cyt. b) is a fragment in the genome which is used to determine the relationship of the species of the same genus or family. While the region Displacement loop (D-loop) is used to determine the degree of kinship intraspecies because two areas, namely HVS-I (Hypervariable Segments I) and HVS-II, have a high mutation (Widayanti 2006). This research aims to (1) to analyze the genetic diversity of genetic markers of Cyt. b, D-loop and HVS-I in the Sumatran tiger; and (2) to determine the relationship of kinship between individuals of Sumatran tiger and between the subspecies of Sumatran tiger with other subspecies in the world.

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