IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Shehzad, W. | |
| Contribution to the development of a non-invasive genetic system for identifying Eurasian lynx (_Lynx lynx_) individuals in France | |
| Full Book | |
The objective of this study is to develop a methodology by using hair, faeces, or tissue carcass from lynx as a source of genetic information. Such a non-invasive approach has already been applied successfully in other carnivores (wolves, bears) but not for the lynx in France.We analysed 69 (2 / 3 tissue carcass and 1 / 3 of faeces) samples collected over a period of 20 years, by using 10 microsatellite markers developed primarily for domestic cat (_Felis catus_), in addition to 2 sex markers. The results obtained show the structure of lynx population into two distinct clusters with a large number of hybrid individuals. In this context, we have relatively low discriminating power to identify individuals from siblings (PIsib).Low amplification rate (4%) was observed from the genotyping of faeces, indicates that our selected markers may not be most specific for the amplified DNA array of lesser quality. So we recommend to analyse more microsatellite markers, in addition to re-design primers to shorten as much as possible the length of the PCR products, which would allow to work with degraded DNA. It would be desirable to design for each locus an internal fluorescent primer (hemi-nested PCR) to limit the amplification of non-specific products |
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