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Queney, G.; Delattre, D.; Fontbonne, A.; Routier, J.-Y.; Lafrance, B.
Establishment of Genetic Profiles in Cheetahs Using Microstatellites [Microsatellites] Developed in the Domestic Cat
2007  Conference Proceeding

Non-invasive techniques of assisted reproduction may be useful in order to provide complementary tools that may help, in specific cases, to restore connectivity among isolated and distant populations, hence reducing the risk of inbreeding depression. For this purpose, genetic profiles have been established in cheetahs in order to optimise natural matings or artificial inseminations with the highest genetic heterogeneity as possible. The genetic prints have been obtained from an 8 microsatellites panel already developed and commonly used in the domestic cat. Genetic profiles have been obtained from 17 cheetahs of different origin: 9 in South Africa (6) and French zoos (3) and 8 in Djibouti. The results show a mean polymorphism of 5.5 allels per marker (2 to 8) and a strong genetic structuration among sub-species. 12 specific allels were identified in the South-African sub-population (out of a total of 30 allels) and 11 specific allels were identified in the Djibouti sub-population (out of a total of 29 allels). The allelic diversity and the heterozygosity calculated from these microsatellites markers are comparable with what is seen among different breeds in the domestic cat.

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