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Kurushima, J.D.; Collins, J.A.; Well, J.A.; Ernest, H.B.
Development of 21 microsatellite loci for puma (_Puma concolor_) ecology and forensics
2006 Molecular Ecology (6): 1260-1262
We developed 21 microsatellite (13 dinucleotide and 8 tetranucleotide) primers specifically for pumas (_Puma concolor_). The primers were tested across 243 individuals from California and Nevada, and displayed an average of 5.5 alleles per locus. Previously, domestic cat (_Felis catus_) primers have been adapted for puma genetic studies. Puma-specific loci may reduce concerns associated with ascertainment bias, improve genetic structure resolution and provide additional tetranucleotide loci valuable for forensic applications. These pumaspecific microsatellites will aid studies involving parentage, kinship and population structure, along with forensic case applications including poaching and puma-associated injuries to humans and domestic animals.
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