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Tolentino, N.; Pinheiro, G.R.G.; Ottino, J.; de Oliveira, A.R.; Coelho, C.M.; Tinoco, H.P.; Fujiwara, R.T.; Santos, R.L.; Ribeiro, V.M.
Serological evidence of _Leishmania _infection by employing ELISA and rapid tests in captive felids and canids in Brazil
2019  Veterinary Parasitology: Regional Studies and Reports (17): 1-5

Visceral leishmaniasis (VL) is a zoonosis and the dog is considered the most important urban reservoir. Cases in cats have been reported, but little is known about _Leishmania _infection and disease in wild felids and canids kept in captivity in endemic areas. Thus, the serological pattern of wild felids and canids kept in captivity at the Belo Horizonte Zoological Garden was investigated using two primary antigens for conventional ELISA: k39 and rKDDR, as well as three serological rapid kits: Dual Path Platform (DPP©) immunochromatographic test, rKDDR Immunochromatographic assay and ELISA SNAP _Leishmania _IDEXX©. A total of 21 serum samples, 13 of wild felids and 8 wild canids of varying age and sex were evaluated. The results obtained in the tests were analyzed by agreement using Kappa coefficient, and between ELISA antigens all the analysis performed had showed significant agreement among both of them, as well between the three immunochromatographic tests. The results demonstrated that there is serological evidence of wild animals seropositive for _Leishmania _antibodies at the Belo Horizonte Zoological Garden, and that all the antigens and rapid tests used can be employed in serological screening for VL in wild felids and canids.

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