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Tinasi, A.L.S.N.; Couto, L.A.; Couto, C.M.V.; Bruno, H.; Junior, P.M.G.
Genetic analysis of hybridization in cats
2019  Nucleus Animalium (11): 49-59

This work was developed at Dr. Francisco Maeda - FAFRAM campus in the city of Ituverava, SP, aimed to elaborate a protocol for analysis of crossbreeding occurrence between domestic and wildcat species, and also to scientifically confirm this occurrence which still non-existent in Brazil. Genetic material of two litters of cats supposedly descending from natural crossing between domestic female cats and a male wildcat were analyzed. For such analysis, two markers associated with the Y chromosome were employed, SMCY and SRY, intending to identify the paternal lineage, and an ATP6 mitochondrial marker, to identify the maternal lineage. The results indicated that the used markers were adequate and sufficient to confirm or to exclude the occurrence of hybridization, in both paternal and maternal lineages, and could be used as protocol for such analysis. On the other hand, this work concluded that the animals belonged to the domestic cat species (_Felis silvestris catus L._), discarding any possibility of hybridization with wildcats of the Brazilian fauna.

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