IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Arantes, L.G. | |
| Effects of cryopreservation on the viability of fibroblasts of wild cats | |
| 2018 Full Book | |
Cryopreservation of genetic material from wild animals, known as germplasm banks, are a viable form of species conservation and biological diversity safeguarding measure. Development of suitable cryopreservation protocols for each cell type is highly necessary considering genetic information banks. This study aims to determine which protocol is best at maintaining cell viability on fibroblasts of the following species; Jaguar (_Panthera onca_), Northern Tiger Cat _(Leopardus tigrinus_) and Pampas Cat (_Leopardus colocolo_) and characterize these cells morphologically. Morphology was examined under Light microscopy, Transmission Electron Microscopy and Scanning Electron Microscopy. Fibroblasts were isolated from skin biopsy and were already cryopreserved on liquid nitrogen (- 196§C). After thawing, the growth curve was determined for growth potential in eight days. None of the species reached confluence before the eighth day. Cultured cells presented exponential growth until the first 50 hours. After this period, reduction of growth speed was noted. Microscopy analysis made possible to note variation between the three wild cat's fibroblasts. _L. colocolo_ cells are fusiform and presents several cytoplasmic projections common to fibroblasts._ L. tigrinus_ cells are more spherical with short ramifications. _P onca_ fibroblasts are fusiform with longer ramifications. Using Transmission Electron Microscopy, it is noted that digestive vesicles, vacuoles and secretion granules are common, an indicative of intense cellular activity. In relation to cryopreservation, there were no significative differences between the concentrations of, 2.5%, 5% and 10% of DMSO for the _L. colocolo_ and _L. tigrinus_, both concentrations being efficient in cryoprotecting the fibroblasts from these species. On the other hand, _P. onca_ fibroblasts, were better preserved with solutions containing 2.5% and 10% DMSO. CryoSOfreeT was efficient to cryopreserve _L. colocolo_ and _L. tigrinus_ fibroblasts. Protocol enhancement will provide the necessary conditions to reproduction for species of interest both on local native fauna and worldwide fauna. Academic studies are also directly enhanced by optimization of such protocols. |
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