IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Suleman, E.; Mtshali, M.S.; Lane, E. | |
| Investigation of false positives associated with loop-mediated isothermal amplification assays for detection of _Toxoplasma gondii_ in archived tissue samples of captive felids | |
| 2016 Journal of Veterinary Diagnostic Investigation (28): 536-542 | |
_Toxoplasma gondii_ is a ubiquitous protozoan parasite that infects humans and many different animals, including felids. Many molecular and serologic tests have been developed for detection of _T. gondii_ in a wide range of hosts. Loop-mediated isothermal amplification (LAMP) is a field-friendly technique that lacks the practical drawbacks of other molecular and serologic tests, and LAMP assays have been successfully developed for detection of _T. gondii_ in fresh tissue samples. In the current study, both a previously published and a de-novo designed LAMP assay were compared to a quantitative real-time (q)PCR assay, for the detection of _T. gondii_ in archived formalin-fixed, paraffin-embedded (FFPE) tissue samples from captive wildlife. The LAMP assays produced conflicting results, generating both false positives and false negatives. Furthermore, the LAMP assays were unable to positively identify samples with low levels of parasites as determined by qPCR and histopathology. Therefore, these LAMP assays may not be the most suitable assays for detection of _T. gondii_ in archived FFPE and frozen tissue samples. |
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