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Srisamoot, N.; Chaveerach, A.; Nuchadomrong, S.; Sattayasi, N.; Chaveerach, P.; Tanomtong, A.; Pinthong, K.
Genetic relationships among wild Felidae in Thailand using AFLP markers
2007  Pakistan Journal of Biological Sciences (10): 2639-2645

The cytogenetics of eight Felidae species in Thailand were investigated by the colchicines-hypotonic fixation-air drying technique followed by a conventional technique. All species studied have an identical number of 38 diploid chromosomes, indicating a close genetic relationship among species. At a deep study level, the genetic relationships of eight Felidae species were accessed by the AFLP method. Blood samples were collected from sources locating in their original regions for DNA extraction. With ten successful primer combinations, a total of 4208 scorable bands were generated. Of these bands, 18.91% are polymorphic. Percentages of Polymorphic Bands (PPB) for each primer combination range from 15.00 to 23.59%. The generating bands were used for dendrogram construction. The average genetic similarity values among all Felidae species are 68.20% (between _Panthera tigris_ and _Neofelis nebulosa_) to 85.53% (between _Prionailurus bengalensis_ and _Prionailurus viverrinus_). The dendrogram shows that the eight Felidae species were clustered together and the subfamily Pantherinae and Felinae with _Neofelis nebulosa_ are distinguished. The Felinae, _Prionailurus bengalensis_, _Prionailurus viverrinus_, _Catopuma temminckii_, _Felis chaus_, _Pardofelis marmorata_ and _Neofelis nebulosa_ were clustered together with 91% bootstrap support and the Pantherinae, _Panthera pardus_ is clustered with _Panthera tigris_ with 92% bootstrap support. In summary, the ten successful primer combinations can be used to determine genetic differences among eight Thailand Felidae species.

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