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| Ijaz, U.; Tahir, M.S.; Majeed, K.A.; Iqbal, S.; Huma, I.; Firyal, S.; Ahmed, I.; Chohan, S.; Khan, A.R. | |
| Molecular characterization and phylogeny of _Panthera pardus_ in Pakistan | |
| 2017 Pakistan Journal of Zoology (49): 67-71 | |
The present study was designed to investigate the evolutionary relationship of the _Panthera pardus _subspecies found in Pakistan by exploring the partial DNA sequences of mitochondrial Cytochrome c Oxidase subunit I (COI) gene. Scat samples of 15 different known leopards were collected from Nathiagali, Khyber Pakhtunkhwa, Pakistan and Azad Jammu and Kashmir, Pakistan. After amplification with specific oligos, the amplicons of partial region of COI were subjected to sequencing, and then observed for single nucleotide polymorphisms (SNPs). SNPs were observed on three loci of the COI gene as compared to reference sequence of _Panthera pardus pardus_. The specimens found in Pakistan were found to be closely related to _Panthera pardus orientalis _(Amur Leopard in South eastern Russia) and _Panthera pardus japonensis _(North Chinese Leopard), with all of these three speculated to be evolved from _Panthera pardus pardus _in not-so-far past. Our findings are helpful in ascertaining the origin and closeness of leopard subspecies in Pakistan. |
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