IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Grisolia, A.B.; Moreno, V.R.; Campagnari, F.; Milazzotto, M.P.; Garcia, J.F.; Adania, C.H.; Souza, E.B. | |
| Genetic diversity of microsatellite loci in _Leopardus pardalis_, _Leopardus wiedii_ and _Leopardus tigrinus_ | |
| 2007 Genetics and Molecular Research (6): 382-389 | |
The microsatellite loci FCA045, FCA077, FCA008, and FCA096 are highly variable molecular markers which were used to determine the genetic diversity in 148 captive _Leopardus _sp. The PCRamplified products of microsatellite loci were characterized in ABI Prism 310 Genetic Analyzer. Allele numbers, heterozygosity, polymorphism information content, exclusive allele number, and shared alleles were calculated. Sixty-five alleles were found and their sizes ranged from 116 to 216 bp in four microsatellite loci. The heterozygosity ranged from 0.36 to 0.81 in _Leopardus pardalis_, 0.57 to 0.67 in _L. tigrinus _and 0.80 to 0.92 in _L. wiedii_. The polymorphism information content was from 0.80 to 0.88 in _L. pardalis_, 0.76 to 0.88 in _L. tigrinus _and 0.77 to 0.90 in _L. wiedii_. The margay (_L. wiedii_) showed the highest index of polymorphism among the three species in this study. These results imply that microsatellite DNA markers can help in the study of the genetic diversity of _Leopardus _specimens. |
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