IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Eo, S.H.; Ko, B.J.; Lee, B.-J.; Seomun, H.; Kim, S.; Kim, M.-J.; Kim, J.H.; An, J. | |
| A set of microsatellite markers for population genetics of leopard cat and cross-species amplification in other felids | |
| 2016 Biochemical Systematics and Ecology (66): 196-200 | |
We describe the isolation and characterization of novel microsatellite loci from the leopard cat, _Prionailurus bengalensis_ Kerr, 1792 (Family Felidae). Using Illumina HiSeq2500 sequencing technology, we sequenced the leopard cat genome and identified 1.5 million loci of simple sequence repeats with di- to deca-nucleotide motifs. We developed twelve polymorphic markers with tetra-nucleotide motif types after screening 35 loci for amplification and polymorphism. The observed and expected heterozygosities of the markers were 0.438 and 0.423, respectively. The number of alleles per locus ranged from 2 to 7, with a mean polymorphism information content of 0.383. Eleven loci were at Hardy-Weinberg equilibrium and no linkage disequilibrium was detected among any pairs of loci. We tested cross-species amplification of these markers across five other felids (_Panthera tigris, P. pardus, P. onca, Acinonyx jubatus_, and _Felis catus_). All loci were transferable to at least one other feline species and four amplified all five species. The microsatellite markers developed in this study will be valuable for estimating ecological parameters of populations and to establish conservation and management strategies for feline species. |
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