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DeCandia, A.; Gaughran, S.; Cragiulo, A.; Amato, G.
A novel molecular method for noninvasive sex identification of order Carnivora
2016 Conservation Genetics Resources (8): 119-121
Noninvasive sampling can provide an efficient means of genetically monitoring mammals. Due to the fragmented quality of DNA derived from such samples, few methods span taxonomic groups beyond species. Here, we describe a more universal protocol for the molecular sex identification of order Carnivora. PCR amplification of a 176 bp segment of the SRY gene indicates a male, whereas no amplification indicates a female. A 245 bp segment of the ZFX gene serves as a positive control. This method is internally consistent, and multiple trials confer confidence in results. It applies to at least three families (Canidae, Felidae, and Phocidae) in both carnivore suborders (Feliformia and Caniformia), rendering this protocol the first sex identification method with amplicon sizes appropriate for noninvasive samples suitable for multiple families within order Carnivora.
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