IUCN / SSC Cat Specialist Group - Digital Cat Library |
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| Metzger, B. | |
| Diagn¢stico de hemoparasitas em fel¡deos neotropicais provenientes de vida livre no Brasil | |
| 2009 Full Book | |
The aim of this study was to investigate the occurrence of _Hepatozoon _spp., piroplasms and hemoplasms in wild caught neotropical felids. Blood samples were drawn from 11 _Leopardus pardalis_, 10 _Leopardus tigrinus_, eight _Puma yagouaroundi, _one _Leopardus wiedii _and one _Puma concolor _coming from the States of Para, MaranhÆo, Piau¡, Cear and Minas Gerais. Blood smears were evaluated for parasites and DNA was extracted for molecular techniques. _Hepatozoon _spp. and piroplasms were diagnosed by Polymerase Chain Reaction (PCR) and feline hemoplasms were examined by both RFLP-PCR and Nested-PCR. Molecular characterization of the isolates obtained was performed after sequencing and phylogenetic analyses. The occurrence of at least one hemoparasite diagnosed by blood smear technique was 6.45% (2/31) and by PCR was 45.16% (14/31). Infections of _Hepatozoon _sp., _Cytauxzoon felis _and feline hemoplasms (from species _Mycoplasma haemofelis _and/or '_Candidatus _Mycoplasma haemominutum') were observed, respectively, in 16.12% (5/31), 3.22% (1/31) and 38.70% (12/31) from the total of animals studied. Infections and co-infections were found among felids. The positive diagnosis were higher in adults (92.85%) (p= 0,007) and in _L. pardalis _(57.14% - 8/14). The detection of _C. felis _in a juvenile, recently wild caught _P. concolor_, indicates the presence of this piroplasm in the wild felid population of Brazil. The molecular characterization of _Hepatozoon _sp. in neotropical felids and the report of this hemoparasite infection in _L. tigrinus _as well as the report of _M. haemofelis _and '_Candidatus _M. haemominutum' in _P. yagouaroundi _are novelties in the world. |
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