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Sachdev, M.
Study of MHC polymorphism in Asiatic lions through molecular tools
2003  Full Book

A study on immune loci polymorphism, particularly at MHC class I in Asiatic lions may provide clues regarding the vulnerability and survivability of these endangered wild cats to various diseases and other stresses and thus may pave the way for their scientific management in both wild and captivity. Hence, the present study was undertaken to have a detailed analysis of polymorphism at MHC loci in Asiatic lions through various molecular tools in order to verify the observations of O'Brien as well as Lalji Singh and group at CCMB, Hyderabad, India. The present study has been designed with the following objectives: 1 To design consensus PCR primers for amplification of MHC loci in Asiatic lions, tigers, leopards, domestic cats, dogs and humans and to amplify the different MHC alleles using these primers. 2.To study the polymorphism at MHC loci in Asiatic lions through PCR based RFLP (PBR). 3.To study the polymorphism at MHC loci in Asiatic lions through Single stranded conformational polymorphism (SSCP). 4.To analyze the MHC loci polymorphism in wild Asiatic lions and compare the degree of polymorphism in Afro-Asian hybrid lions and captive lions through sequence based typing. 5. To analyze the MHC loci polymorphism at nucleotide level in a representative set of tigers, leopards, domestic cats, dogs and humans through sequence based typing. 6.To correlate the MHC polymorphism data of Asiatic lions with their management and breeding strategies.

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